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Image Search Results
Journal: The Journal of Experimental Medicine
Article Title: Triggering CD40 on endothelial cells contributes to tumor growth
doi: 10.1084/jem.20060844
Figure Lengend Snippet: Angiogenic effect of CD40 in vivo. Liquid growth factor–reduced Matrigel, containing Ab to CD40, isotype-matched control Ab, sCD40L plus enhancer, bFGF (as positive control), or without any additives, was injected s.c. in BALB/c or CD40-KO mice. After 7–10 d, semisolid plugs were recovered and included in paraffin or OCT. (A) Hematoxylin and eosin staining of Matrigel sections in BALB/c and CD40-KO mice. Bar, 500 μm. (B) Percentage of CD31 + cells over total number of cells in the Matrigel plugs in BALB/c and CD40-KO mice (mean of 10 fields/sample). (C) Immunostaining of Matrigel plug sections with Ab to CD31. Black arrows indicate the edge of the Matrigel plug. Bar, 300 μm; bar in inset, 300 μm.
Article Snippet: Matrigel plug assay has been performed in BALB/c and CD40-KO mice by s.c. injection of growth factor–reduced
Techniques: In Vivo, Positive Control, Injection, Staining, Immunostaining